|
| Status |
Public on May 05, 2022 |
| Title |
XCR1-DTR DTx + aPD-L1 (D11_3) |
| Sample type |
SRA |
| |
|
| Source name |
CD8+, PD-1+ CD8 T cell from spleen
|
| Organism |
Mus musculus |
| Characteristics |
strain: XCR1-DTR (Xcr1tm2(HBEGF/Venus)Ksho)
tissue: spleen
genotype: XCR1-DTR heterozygous
treatment: DTx + aPD-L1 (96h)
|
| Treatment protocol |
B/6 Mice were infected with 2x10^6 IU LCMV clone13 after 2 times CD4 T cell depletion (d-2 and d0) with 300µg GK1.5 antibody. At the chronic infection phase (> d24), splenocytes were isolated and CD8+, PD-1+, Tim-3 low (F7 + C1_2) or CD8+, PD-1+ (D11_3 + D12_3) cells were sorted using FACS AriaIII. Treated mice were injected i.p. 200µg aPD-L1 antibody (clone 10F.9G2) 2h before cell isolation or 2 times with 200µg aPD-L1 antibody (clone 10F.9G2) and 500ng DTX before cell isolation. Cells were pooled form 3-4 mice per group.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Not provided
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
The sequencing data was processed using CellRanger software.
|
| |
|
| Submission date |
Dec 20, 2021 |
| Last update date |
May 05, 2022 |
| Contact name |
Antoine-Emmanuel Saliba |
| E-mail(s) |
emmanuel.saliba@helmholtz-hzi.de
|
| Phone |
+49-931-31-81341
|
| Organization name |
Helmholtz Institute for RNA-based Infection Research
|
| Street address |
Josef-Schneider-Straße 2 / D15
|
| City |
Würzburg |
| ZIP/Postal code |
97080 |
| Country |
Germany |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE168282 |
cDC1 retain a niche for precursor exhausted T cells during chronic viral infection |
|
| Relations |
| BioSample |
SAMN24250987 |
| SRA |
SRX13458661 |
