|
| Status |
Public on Dec 17, 2021 |
| Title |
Mycn mutant zebrafish embryos 72 hpf |
| Sample type |
SRA |
| |
|
| Source name |
zebrafish cells
|
| Organism |
Danio rerio |
| Characteristics |
strain: Mycn mutant
tissue: embryonic cells
age: 72 hpf
|
| Treatment protocol |
WT and Mycn mutant zebrafish embryos were collected for RNA sequencing at 72 hpf
|
| Growth protocol |
embryos were cultured in a Petri dish filled with 0.3X Danieau buffer
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Libraries were prepared using Chromium Controller and Chromium Single Cell 3’Library & Gel Bead Kit v3 (10x Genomics, PN-1000074) according to the manufacturer’s protocol for 10000 cells recovery.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Illumina sequencing reads were aligned to the zebrafish mRNA reference genome (GRCz11) using the 10x Genomics CellRanger pipeline (version 3.0.2) with default parameters.
Genome_build: GRCz11
Supplementary_files_format_and_content: tar archive included filtered_gene_bc_matrices after running CellRanger pipeline
|
| |
|
| Submission date |
Dec 15, 2021 |
| Last update date |
Dec 17, 2021 |
| Contact name |
Tao cheng |
| E-mail(s) |
11818118@zju.edu.cn
|
| Phone |
0571-88208276
|
| Organization name |
Zhejiang University
|
| Department |
Institute of genetics
|
| Street address |
886 Yuhangtang Road
|
| City |
Hang Zhou |
| State/province |
Zhe Jiang |
| ZIP/Postal code |
310000 |
| Country |
China |
| |
|
| Platform ID |
GPL24995 |
| Series (2) |
| GSE191002 |
Mycn regulates intestinal development through ribosomal biogenesis in a zebrafish model of Feingold syndrome 1 [single-cell RNA-seq] |
| GSE191003 |
Mycn regulates intestinal development through ribosomal biogenesis in a zebrafish model of Feingold syndrome 1 |
|
| Relations |
| BioSample |
SAMN24104936 |
| SRA |
SRX13418942 |
