|
| Status |
Public on Dec 31, 2022 |
| Title |
IL-15Tg-Xbp1s cKO 2 |
| Sample type |
SRA |
| |
|
| Source name |
mouse immune cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: mouse NK cells
strain: C57BL/6
tissue: spleen
genotype/variation: IL-15Tg-Xbp1s cKO
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from purified splenic NK cells from IL-15Tg-WT and IL-15Tg-Xbp1s cKO mice using a RNeasy Mini Kit (QIAGEN).
The RNA libraries were generated using the KAPA Hyper Prep Kit (KAPA Biosystems). Sequencing was performed on the HiSeq2500. Sequencing was performed at the City of Hope Genomics Facility on an Illumina HiSeq2500 machine with single read 50 bp mode.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Sequencing reads were mapped to the mouse genome using HISAT2 (Version: v101)
Transcript counts were generated using HTSeq and differential gene analysis was performed using DESeq2.
Genome_build: mm10
Supplementary_files_format_and_content: RPKM values
|
| |
|
| Submission date |
Nov 15, 2021 |
| Last update date |
Dec 31, 2022 |
| Contact name |
Shoubao Ma |
| E-mail(s) |
shma@coh.org
|
| Phone |
6262182182
|
| Organization name |
City of Hope
|
| Street address |
1500 East Duarte Road
|
| City |
duarte |
| State/province |
California |
| ZIP/Postal code |
91010 |
| Country |
USA |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE188796 |
Xbp1s controls IL-15-mediated natural killer cell survival and function by regulating Pim2 and Tbet |
|
| Relations |
| BioSample |
SAMN23139443 |
| SRA |
SRX13138323 |
