|
| Status |
Public on Jul 20, 2010 |
| Title |
skin, 12hrs post-wounding, biological replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
skin, 12hrs post-wounding
|
| Organism |
Mus musculus |
| Characteristics |
strain: Balb/c
gender: female
age: 6-8 weeks
tissue: skin
time post-wound: 12 hrs
|
| Biomaterial provider |
Harlan Inc.
|
| Treatment protocol |
2mm biopsies were collected at defined time intervals after wounding (6hrs, 12hrs, 24hrs, 3days, 5days, 7days, 10days) and from unwounded tissue.
|
| Growth protocol |
n/a
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The RNeasy total RNA isolation protocol (Qiagen) was utilized.
|
| Label |
biotin
|
| Label protocol |
Standard 3' IVT target labeling protocol (Affymetrix) starting with 5 micrograms of total RNA per labeling reaction.
|
| |
|
| Hybridization protocol |
15 micrograms of fragmented cRNA were hybridized overnight at 45C, and washed and stained according to the EukGE-WS2v5_450 Affymetrix protocol.
|
| Scan protocol |
Arrays were scanned using Affymetyrix GeneChip Scanner 3000 7G.
|
| Description |
LAD111607_12s2
|
| Data processing |
Hybridization intensities were normalized by quantiles and summarized using the RMA method. The S+ArrayAnalyzed statistical package (Insightful) was used for data transformation and subsequent statistical analysis.
|
| |
|
| Submission date |
Jul 19, 2010 |
| Last update date |
Jul 19, 2010 |
| Contact name |
Zarema H Arbieva |
| E-mail(s) |
zarbieva@uic.edu
|
| Phone |
312-355-1608
|
| Organization name |
UIC
|
| Department |
RRC
|
| Lab |
Core Genomics Laboratory
|
| Street address |
835 S. Wolcott A301
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60612 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE23006 |
Transcriptional profiling of a wound healing process in skin and oral mucosa |
|
