|
| Status |
Public on Jul 21, 2010 |
| Title |
Human acute myeloid leukaemia cell line THP-I.2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
THP-I, MSI2 shRNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: THP-I
treatment: MSI2 shRNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Quick Amp Agilent protocol
|
| |
|
| Channel 2 |
| Source name |
THP-I, control
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: Control
cell line: THP-I
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
Quick Amp Agilent protocol
|
| |
|
| |
| Hybridization protocol |
Agilent protocol
|
| Scan protocol |
Agilent protocol
|
| Description |
Scramble Control shRNA (Cy5) vs msi2 shRNA hairpin2 (Cy3), THP-I, dye-swap
|
| Data processing |
Image intensity data were recorded and transferred into text files using Feature Extraction software (version 8.5). Limma R/BioC package was used for background correction. Loess normalization was applied within arrays and between arrays using a quantile normalization procedure, and log transformed
|
| |
|
| Submission date |
Jul 06, 2010 |
| Last update date |
Jul 21, 2010 |
| Contact name |
Fatima Al-Shahrour |
| E-mail(s) |
shahrour@broadinstitute.org
|
| Organization name |
Broad Institute of MIT and Harvard
|
| Department |
Cancer program
|
| Street address |
7 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL4133 |
| Series (2) |
| GSE22775 |
Musashi 2 regulates normal hematopoiesis and accelerates leukemogenesis (Leukemia cell lines) |
| GSE22778 |
Musashi 2 regulates normal hematopoiesis and accelerates leukemogenesis |
|
