|
Status |
Public on Aug 18, 2010 |
Title |
Treg_control |
Sample type |
SRA |
|
|
Source name |
CD4+CD25+ Treg
|
Organism |
Mus musculus |
Characteristics |
genetic background: C57BL/6 backcrossed sample type: FACS-sorted from spleen and lymph nodes of CD4-cre mice age: 6-7 weeks cell type: CD4+CD25+ Treg
|
Extracted molecule |
total RNA |
Extraction protocol |
19-30nt small RNAs fractionated by gel electrophoresis. Adaptors were ligated only to species with 5' phosphate and 3' hydroxyl
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
size fractionation |
Instrument model |
Illumina Genome Analyzer II |
|
|
Description |
Adaptor sequences clipped then mapped to miRNA loci
|
Data processing |
Sequences were first clipped of 3' adaptor sequencess (5'-CTGTAGGCACCATCAAT) as small RNAs are shorter than the 36bp read lengths. These were then mapped to the mouse genome.
|
|
|
Submission date |
Jul 06, 2010 |
Last update date |
May 15, 2019 |
Contact name |
Mark Chong |
E-mail(s) |
mchong@svi.edu.au
|
Phone |
61-3-92313444
|
Organization name |
St Vincent's Institute of Medical Research
|
Street address |
9 Princes Street
|
City |
Fitzroy |
State/province |
Victoria |
ZIP/Postal code |
3065 |
Country |
Australia |
|
|
Platform ID |
GPL9250 |
Series (1) |
GSE22760 |
Small RNA profiling of RNase III enzyme deficient DN3 thymocytes, Tregs, activated CD4+ T cells, and embryonic fibroblasts |
|
Relations |
SRA |
SRX023160 |
BioSample |
SAMN00016939 |