|
| Status |
Public on Aug 18, 2010 |
| Title |
Treg_control |
| Sample type |
SRA |
| |
|
| Source name |
CD4+CD25+ Treg
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: C57BL/6 backcrossed
sample type: FACS-sorted from spleen and lymph nodes of CD4-cre mice
age: 6-7 weeks
cell type: CD4+CD25+ Treg
|
| Extracted molecule |
total RNA |
| Extraction protocol |
19-30nt small RNAs fractionated by gel electrophoresis. Adaptors were ligated only to species with 5' phosphate and 3' hydroxyl
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
Adaptor sequences clipped then mapped to miRNA loci
|
| Data processing |
Sequences were first clipped of 3' adaptor sequencess (5'-CTGTAGGCACCATCAAT) as small RNAs are shorter than the 36bp read lengths. These were then mapped to the mouse genome.
|
| |
|
| Submission date |
Jul 06, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Mark Chong |
| E-mail(s) |
mchong@svi.edu.au
|
| Phone |
61-3-92313444
|
| Organization name |
St Vincent's Institute of Medical Research
|
| Street address |
9 Princes Street
|
| City |
Fitzroy |
| State/province |
Victoria |
| ZIP/Postal code |
3065 |
| Country |
Australia |
| |
|
| Platform ID |
GPL9250 |
| Series (1) |
| GSE22760 |
Small RNA profiling of RNase III enzyme deficient DN3 thymocytes, Tregs, activated CD4+ T cells, and embryonic fibroblasts |
|
| Relations |
| SRA |
SRX023160 |
| BioSample |
SAMN00016939 |
