|
| Status |
Public on Oct 01, 2010 |
| Title |
OP9 stromal cell |
| Sample type |
RNA |
| |
|
| Source name |
Calvaria of newborn B6C3Fe-a/a-Csf1op/Csf1op mouse
|
| Organism |
Mus musculus |
| Characteristics |
cell line: OP9 stromal cell line
protocol: control
|
| Treatment protocol |
None.
|
| Growth protocol |
Cells were maintained with aMEM supplemented with 20% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA samples were extracted from cells using RNesy mini kit (Quiagen).
|
| Label |
Cy5
|
| Label protocol |
Amino Allyl aRNA was synthesis by Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion#1753). CyeDye Coupling and fragmentation were performed as the protocol supplied by TORAY Industries, Inc..
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 37 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
|
| Scan protocol |
ScanArray Express (PerkinElmer Japan Co., Ltd.) was used for scanning. Images were quantified using GenePix Pro version 6.0(Axon Instruments).
|
| Description |
OP9
|
| Data processing |
F635 Median column as Red signal. The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals.
|
| |
|
| Submission date |
Jul 01, 2010 |
| Last update date |
Jul 01, 2010 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Department |
New Projects Development Division
|
| Street address |
Tebiro 6-10-1
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL5642 |
| Series (1) |
| GSE22659 |
A Notch ligand, Delta-like 1 functions as an adhesion molecule for mast cells |
|
