|
| Status |
Public on Jul 02, 2010 |
| Title |
HDF_B |
| Sample type |
RNA |
| |
|
| Source name |
Human dermal fibroblast line HDF_B replicate 2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Human dermal fibroblast line HDF_B
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using the MirVana RNA extraction kit (Ambion), quantified using the Ribogreen reagent (Lifetech, Inc.), and quality-controlled on a Bioanalyzer (Agilent). Two hundred nanograms of input total RNA was amplified and labeled using the TotalPrep kit (Ambion). The labeled product was then hybridized to Illumina HT12 arrays and scanned on a BeadArray Reader (Illumina, Inc.) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Human dermal fibroblast line HDF_B replicate 2
|
| Data processing |
The data were normalised using quantile normalisation with Illumina quantile normalization in Genome Studio.
|
| |
|
| Submission date |
Jun 30, 2010 |
| Last update date |
Jul 01, 2010 |
| Contact name |
JOEL GOTTESFELD ; JEANNE LORING ; GULSAH ALTUN |
| E-mail(s) |
joelg@scripps.edu, skusen@home2.scripps.edu, jloring@scripps.edu, gulsah@scripps.edu, gulsahaltun@gmail.com
|
| Phone |
8587847753
|
| Organization name |
THE SCRIPPS RESEARCH INSTITUTE
|
| Department |
CHEMICAL PHYSIOLOGY
|
| Lab |
JEANNE LORING
|
| Street address |
3030 Science Park Rd. 3rd floor, room 3020
|
| City |
SAN DIEGO |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE22651 |
Friedreich’s Ataxia Induced Pluripotent Stem Cells Recapitulate GAA•TTC Triplet-Repeat Instability |
|
