|
| Status |
Public on Apr 24, 2022 |
| Title |
Sg_Smarcd2_rep4 |
| Sample type |
RNA |
| |
|
| Source name |
sgRNA-transduced OT-1 cells from the dual-color transfer system at day 7.5 post-infection from spleen
|
| Organism |
Mus musculus |
| Characteristics |
cell line: OT-I
treatment: Sg_Smarcd2
|
| Treatment protocol |
Sorted cells were lysed with lysis buffer from Rneasy Micro kit from Qiagen.
|
| Growth protocol |
Specifically, OT-I cells were transduced with non-targeting control (NTC) single-guide RNA (sgNTC) labelled with GFP, or with indicated sgRNA marked by the expression of Ametrine, and then these cells were mixed at a 1:1 ratio and transferred to the same host, followed by Lm-ova infection. Donor-derived CD8+ T cells were sorted at day 7.5 after post-infection. RNA was isolated and loaded on Clariom S mouse array platform.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA samples were prepared according to the prototol of Rneasy Micro kit from Qiagen,
|
| Label |
biotin
|
| Label protocol |
Total RNA (1 - 20 ng) were converted into biotinylated cDNA using the NuGEN WTA Pico v2 protocol and the NuGEN Encore Biotin module
|
| |
|
| Hybridization protocol |
Six micrograms of biotinylated cDNA was fragmented and then hybridized for 16 hr at 45C on an Affymetrix Clariom S mouse GeneChip. Following hybridization, GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the AffymetrixGeneChip Scanner 3000 7G.
|
| Data processing |
Probe signals were quantile normalized and summarized by the RMA algorithm using the Affymetrix Expression Console software v1.1
|
| |
|
| Submission date |
Sep 22, 2021 |
| Last update date |
Apr 26, 2022 |
| Contact name |
Douglas R Green |
| E-mail(s) |
douglas.green@stjude.org
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Immunology
|
| Lab |
Green lab
|
| Street address |
262 Danny Thomas Pl
|
| City |
Memphis |
| State/province |
United States |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL23038 |
| Series (2) |
| GSE183619 |
BAF complex cooperates with c-Myc to orchestrate early fate decisions of CD8 T cells |
| GSE184587 |
BAF complex cooperates with c-Myc to orchestrate early fate decisions of CD8 T cells (Microarray) |
|
