|
| Status |
Public on Jan 07, 2022 |
| Title |
RCC_AJ194: Renal cell carcinoma 2 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
A/J mouse normal kidney
|
| Organism |
Mus musculus |
| Characteristics |
genotype: A/J wild type
Sex: male
tissue: normal kidney
|
| Treatment protocol |
The treated mice were intraperitoneally injected with ferric nitrilotriacetate 5 times a week for 12 weeks. The administration was started at a dose of 5 mg iron/mouse kg weight for the first two weeks. The dose was increased to 7 mg iron/mouse kg weight for the next 10 weeks. The mice were euthanized when they were dying.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using DNeasy Blood & Tissue Kit (QIAGEN).
|
| Label |
Cy3
|
| Label protocol |
The extracted genomic DNA was labeled using Aagilent Sure tag DNA labeling kit according to the manufacturer's protocol (Version 8.0).
|
| |
|
| Channel 2 |
| Source name |
RCC_AJ194
|
| Organism |
Mus musculus |
| Characteristics |
genotype: A/J wild type
Sex: male
treatment: Fe-NTA, 3 months
tissue: renal cell carcinoma
|
| Treatment protocol |
The treated mice were intraperitoneally injected with ferric nitrilotriacetate 5 times a week for 12 weeks. The administration was started at a dose of 5 mg iron/mouse kg weight for the first two weeks. The dose was increased to 7 mg iron/mouse kg weight for the next 10 weeks. The mice were euthanized when they were dying.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using DNeasy Blood & Tissue Kit (QIAGEN).
|
| Label |
Cy5
|
| Label protocol |
The extracted genomic DNA was labeled using Aagilent Sure tag DNA labeling kit according to the manufacturer's protocol (Version 8.0).
|
| |
|
| |
| Hybridization protocol |
The labeled DNA was hybridized with Agilent SurePrint G3 Mouse CGH 4x180k microarray at 67°C for 24 hours according to the manufacturer's protocol (Version 8.0).
|
| Scan protocol |
The slides were scanned in an Agilent DNA microarray scanner with SureScan High-Resolution Technology (G2565CA).
|
| Data processing |
The scanned images were analyzed with Agilent Feature Extraction Software 10.7 using default parameters (CGH_107_Sep09 protocol).
|
| |
|
| Submission date |
Sep 01, 2021 |
| Last update date |
Jan 09, 2022 |
| Contact name |
Shinya Toyokuni |
| E-mail(s) |
akatsuka@med.nagoya-u.ac.jp
|
| Organization name |
Nagoya University
|
| Department |
Pathology
|
| Street address |
65 Tsuruma-Cho, Showa-Ku
|
| City |
Nagoya |
| State/province |
Aichi |
| ZIP/Postal code |
466-8550 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10449 |
| Series (1) |
| GSE183173 |
Genomic profiles of oxidative stress-induced renal cell carcinomas developed in A/J mice |
|
