|
| Status |
Public on Jul 24, 2021 |
| Title |
autoantigen_V2_Patient SU10 V2 |
| Sample type |
protein |
| |
|
| Source name |
Hospitalized COVID-19
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: serum
condition: Hospitalized COVID-19
|
| Extracted molecule |
protein |
| Extraction protocol |
Blood was collected from patients and healthy controls from 3 different centers. The blood was then processed into serum which was then run on our arrays.
|
| Label |
R-PE conjugated goat anti-human IgG secondary antibody
|
| Label protocol |
A secondary antibody conjugated to a fluorophore was added to a PBS solution containing conjugated beads bound to antibodies from serum samples.
|
| |
|
| Hybridization protocol |
A secondary antibody conjugated to a fluorophore was added to a PBS solution containing conjugated beads bound to antibodies from serum samples.
|
| Scan protocol |
A Luminex FlexMAP 3D instrument was used to measure fluorescence (in MFI) from each barcoded bead.
|
| Description |
Antibodies in patient serum samples bind to conjugated beads in protein array. The fluorescence measured as MFI from the secondary antibody was used to determine serum antibody reactivity.
|
| Data processing |
Sample replicate MFI values were averaged for each antigen. The average barebead MFI was then subtracted from the average MFI of each sample and antigen.
|
| |
|
| Submission date |
Jul 23, 2021 |
| Last update date |
Jul 24, 2021 |
| Contact name |
Allan Feng |
| E-mail(s) |
afeng1@stanford.edu
|
| Phone |
2055678956
|
| Organization name |
Stanford School of Medicine
|
| Department |
Imm/Rheum
|
| Lab |
Utz
|
| Street address |
269 Campus Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL30431 |
| Series (2) |
| GSE180738 |
New-Onset IgG Autoantibodies in Hospitalized Patients with COVID-19 [Autoantigen V2] |
| GSE180743 |
New-Onset IgG Autoantibodies in Hospitalized Patients with COVID-19 |
|
