|
| Status |
Public on May 19, 2010 |
| Title |
Bmi1-shRNA 0 hrs (2) |
| Sample type |
RNA |
| |
|
| Source name |
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: RPMI-8226 multiple myeloma cell line
stable infection: Bmi1-shRNA
dox induction: dox
time post-induction: 0 hours
replicate: 2
|
| Treatment protocol |
If dox induction, then shRNAs induced using 100ng/ml doxycline.
|
| Growth protocol |
Cell lines were maintained in Advanced RPMI-8226 medium (Invitrogen) with 1% FBS, and 1% LGlutamine.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNeasy Mini Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 to 5 µg of total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation cRNA was hybridized at 45ºC for approximately 18 hours, arrays were stained on GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
|
| Scan protocol |
GeneChips were scanned using the GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
|
| Description |
RPMI-8226 multiple myeloma cell line with inducible control shRNA
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
|
| |
|
| Submission date |
May 19, 2010 |
| Last update date |
May 19, 2010 |
| Contact name |
Michael Morrissey |
| E-mail(s) |
michael.morrissey@novartis.com
|
| Phone |
617-871-7418
|
| Organization name |
Novartis Institutes for Biomedical Research
|
| Department |
Oncology
|
| Street address |
250 Massachusetts Ave
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE21912 |
The Polycomb Group Protein Bmi-1 is essential for the growth of Multiple Myeloma cells |
|
