|
| Status |
Public on May 18, 2011 |
| Title |
P1-control-ex3 |
| Sample type |
RNA |
| |
|
| Source name |
P1 retinas of control mice
|
| Organism |
Mus musculus |
| Characteristics |
tissue: retina
genotype: otx2 flox/flox; Crx-Cre- (control)
time: P1
|
| Growth protocol |
Mice were housed in a temperature-controlled room with a 12 h light/dark cycle. Fresh water and rodent diet were available at all times.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The mouse retinas were dissected using forceps.
Total RNA (5 ug) of the retina was isolated using TRIzol reagent (Invitrogen) and converted to cDNA using One-Cycle cDNA synthesis kit (Affymetrix) according to the manufacture’s instruction.
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled cRNA was prepared using IVT labeling kit.
|
| |
|
| Hybridization protocol |
cRNA was hybridized to GeneChip mouse genome 430 2.0 arrayin Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000-7G.
|
| Description |
Gene expression data from the Otx2 CKO retina at P1
|
| Data processing |
Signal intensity was determined using GeneChip Operating Software 1.4.
|
| |
|
| Submission date |
May 19, 2010 |
| Last update date |
May 18, 2011 |
| Contact name |
Takahisa Furukawa |
| Organization name |
Osaka Bioscience Institute
|
| Department |
Developmental Biology
|
| Lab |
Furukawa Lab
|
| Street address |
6-2-4 Furuedai
|
| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
565-0874 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE21900 |
Expression profiling of the Otx2 CKO retina |
|
