|
| Status |
Public on Aug 09, 2010 |
| Title |
R1 t=16 [RAS profiling] |
| Sample type |
RNA |
| |
|
| Source name |
KRAS P53LSL Lung Tumor Cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6 x 129SvJ
agent: 4-hydroxytamoxifen
time: 16hrs
|
| Treatment protocol |
For p53 restoration, cultured tumor cell lines were incubated with 500nM 4-hydroxytamoxifen for the indicated times
|
| Growth protocol |
Lung tumor-derived cell lines were isolated from individual tumors from KrasLA2/+; Trp53LSL/LSL Rosa26CreERT2 animals and grown in DMEM + 10%FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Biotin
|
| Label protocol |
Standard Affymetrix protocols
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocols
|
| Scan protocol |
Standard Affymetrix protocols
|
| Description |
Profiling of p53 inducible KRAS Lung Tumors
Cre Treated for 16hrs
|
| Data processing |
RMA analysis was performed using the Bioconductor package
|
| |
|
| Submission date |
May 10, 2010 |
| Last update date |
Aug 09, 2010 |
| Contact name |
Mitchell Guttman |
| E-mail(s) |
mguttman@mit.edu
|
| Organization name |
Broad Institute
|
| Street address |
7 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE21761 |
A large intergenic non-coding RNA induced by p53 mediates global gene repression in the p53 transcriptional response |
|
