|
| Status |
Public on Jul 20, 2023 |
| Title |
HCA2 cells, senescent-2 |
| Sample type |
SRA |
| |
|
| Source name |
HCA2 (MJ90)
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HCA2 (MJ90)
cell type: skin fibroblast
cell condition: RO3306+Nutlin3a treatment
phenotype: senescent
|
| Treatment protocol |
RO3306 (9 uM) + Nulin3a (5 uM)
|
| Growth protocol |
Cells were cultured in DMEM (Invitrogen) supplemented with 10% fetal bovine serum (FBS).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy mini kit (Qiagen) according to the manufacturer’s instructions.
Ion Total RNA-Seq kit v2 (Thermo Fisher Scientific) according to the manufacturer’s instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Ion Torrent Proton |
| |
|
| Description |
S5
|
| Data processing |
The libraries were sequenced on a Ion Proton System platform
The sequencing reads were aligned to the hg19/GRCh37 reference human genome using Bowtie2 (v2.0.0) and STAR (v2.3.0).
Data normalization and differential expression analysis were performed using a edgeR package in the R language (v3.2.2).
Genome_build: hg19
Supplementary_files_format_and_content: Transcriptome among young and senescent fibroblasts.xlsx
|
| |
|
| Submission date |
Jul 05, 2021 |
| Last update date |
Jul 20, 2023 |
| Contact name |
Satotaka Omori |
| E-mail(s) |
satotaka.omori.r@gmail.com
|
| Phone |
0354495449
|
| Organization name |
the university of Tokyo
|
| Street address |
shirokanedai, 4-6-1
|
| City |
Minato ku |
| State/province |
tokyo |
| ZIP/Postal code |
108-0071 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17303 |
| Series (1) |
| GSE179465 |
Transcriptome analysis among young and senescent fibroblasts |
|
| Relations |
| BioSample |
SAMN20062897 |
| SRA |
SRX11354418 |
