|
| Status |
Public on Nov 11, 2021 |
| Title |
mPFC LSD rep6 |
| Sample type |
SRA |
| |
|
| Source name |
mPFC
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
gender: male
tissue: medial prefrontal cortex
treatment: LSD
|
| Treatment protocol |
Rats were injected (i.p.) with either sterile saline, or LSD (0.16 mg/kg) every other day for 90 days.
|
| Growth protocol |
Male Sprague-Dawley rats obtained from Harlan (Indianapolis, IN) were aged 6-8 weeks (180-200 g) at the start of treatment. Animals were housed individually for seven days before treatment, during which time they were handled for 15 min each day. During treatment, rats were individually housed in translucent home cages, allowed free access to food and water, and kept on a 12:12 h light/dark cycle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
4 μg of total extracted RNA were subjected to poly(A) selection.
Sample libraries were prepared with Illumina mRNA sample preparation kits.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Description |
LSD_6
|
| Data processing |
Reads were mapped to the Rattus norvegicus rn6 reference assembly using Hisat2.
Gene counts were computed using the htseq-count tool from the HTSeq package and the ncbi RefSeq gtf file.
Genome_build: rn6 (Rnor_6.0)
Supplementary_files_format_and_content: Raw_gene_counts_matrix.txt: Matrix table with raw gene counts for every gene and every sample.
|
| |
|
| Submission date |
Jul 03, 2021 |
| Last update date |
Nov 11, 2021 |
| Contact name |
Aurora Savino |
| E-mail(s) |
aurora.savino@unito.it
|
| Organization name |
Università di Torino
|
| Street address |
via Nizza, 52
|
| City |
Torino |
| State/province |
Piemonte |
| ZIP/Postal code |
10126 |
| Country |
Italy |
| |
|
| Platform ID |
GPL10669 |
| Series (2) |
| GSE179379 |
Chronic LSD alters gene expression profiles in the mPFC relevant to schizophrenia [RNA-seq] |
| GSE179380 |
LSD influences gene expression patterns within the mammalian brain |
|
| Relations |
| BioSample |
SAMN20054344 |
| SRA |
SRX11345870 |
