|
| Status |
Public on Jul 04, 2024 |
| Title |
24 month hippocampal microglia |
| Sample type |
SRA |
| |
|
| Source name |
Cd11b+ cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: hippocampus
cell type: microglia
age: 24 month
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Hippocampi were dissociated using Miltenyi Neural Tissue Dissociation kit (P) followed by myelin depletion and CD11b cell enrichment using magnetic beads. Gel bead emulsions are generated on the 10x Chromium controller that contain single cells, barcodes, and reverse transcription reagents
10x Single Cell 3' v2
scRNA-Seq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
cellranger mkfastq (version 2.1.1) generated fastq files
cellranger count (version 2.1.1) mapped fastq files to the mm10 transcriptome and created gene expression matrices.
Genome_build: mm10
Supplementary_files_format_and_content: Sparse expression matrix
|
| |
|
| Submission date |
Jul 02, 2021 |
| Last update date |
Jul 04, 2024 |
| Contact name |
Jeremy Michael Shea |
| Organization name |
University of California, San Francisco
|
| Department |
Anatomy
|
| Lab |
Villeda
|
| Street address |
513 Parnassus Avenue
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE179358 |
Microglia aging progresses through functional intermediate steps |
|
| Relations |
| BioSample |
SAMN20013892 |
| SRA |
SRX11343046 |
