|
| Status |
Public on Jul 19, 2010 |
| Title |
hmp257 cell line [mouse mRNA] |
| Sample type |
RNA |
| |
|
| Source name |
mouse tumor model
|
| Organism |
Mus musculus |
| Characteristics |
disease state: mouse ependymoma model
tissue: Spinal
genotype: Arf null
age: adult
|
| Treatment protocol |
tumor exicision or cell line suspension extraction
|
| Growth protocol |
mouse tumors and cell lines
|
| Extracted molecule |
total RNA |
| Extraction protocol |
standard Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix One-Cycle protocol from 1 ug of total RNA (Expression Analysis Technical Manual, 2005-2006, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from mouse model tumor models
|
| Data processing |
The data were analyzed with GCOS v1.4 software (Affymetrix, Santa Clara, CA). Signals were normalized for each array by scaling to a 2% trimmed mean of 500.
|
| |
|
| Submission date |
May 05, 2010 |
| Last update date |
Oct 08, 2010 |
| Contact name |
Richard James Gilbertson |
| E-mail(s) |
richard.gilbertson@stjude.org
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Developmental Neurobiology
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE21687 |
Comparative genomics matches mutations and cells to generate faithful ependymoma models |
|
