|
| Status |
Public on Aug 25, 2021 |
| Title |
cardiac_neural crest cells_1 |
| Sample type |
RNA |
| |
|
| Source name |
E10.5 mouse cardiac neural crest cells
|
| Organism |
Mus musculus |
| Characteristics |
developmental stage: E10.5
tissue: The 3rd, 4th and 6th pharyngeal arches and outflow tract
cell type: cardiac neural crest cells
|
| Treatment protocol |
Collected tissues were digested and sorted for both EGFP+ cranial NCCs and EGFP- control cells by FACS sorting
|
| Growth protocol |
E10.5 Rosa-mT-mG/Wnt1-Cre EGFP positive embryos were collected. The 3rd, 4th and 6th pharyngeal arches and outflow tract were dissected out.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cell total RNA was isolated using Trizol reagent.
|
| Label |
biotin
|
| Label protocol |
Biotinylated RNA were prepared according to the standard GeneChip miRNA Array Target Protocol from 1 ug total RNA
|
| |
|
| Hybridization protocol |
Hybridize arrays at 48ºC for 16 hrs at 60rpm
|
| Scan protocol |
Transfer clean arrays to the Scanner 3000 Autoloader and initiate the scanner using the AGCC Scanner Control functions of Command Console
|
| Data processing |
The microarray signals were analyzed with Affymetrix Expression Console Software
The data were analyzed with Affymetrix Expression Console Software using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jun 24, 2021 |
| Last update date |
Aug 25, 2021 |
| Contact name |
Youchen Yan |
| E-mail(s) |
yanych8@mail2.sysu.edu.cn
|
| Organization name |
The First Affiliated Hospital, Sun Yat-sen University
|
| Street address |
No.58 Zhongshan Road II
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510080 |
| Country |
China |
| |
|
| Platform ID |
GPL8786 |
| Series (1) |
| GSE178823 |
Profiling of microRNA expression of mouse cardiac neural crest cells and non neural crest cells |
|
