|
| Status |
Public on Apr 11, 2011 |
| Title |
breast tissue, Neg Contr., Rep. 5 |
| Sample type |
RNA |
| |
|
| Source name |
breast tissue of WAP-TNP8 mice bevor activation of the SV40 oncogene
|
| Organism |
Mus musculus |
| Characteristics |
strain: WAP-TNP8
tissue: breast
|
| Treatment protocol |
Breast tissue was snap frozen directly after preparation and stores at -80°C until RNA isolation.
|
| Growth protocol |
WAP-TNP8 animals, which selectively synthesize the T/t-antigen under the control of the WAP promoter in mammary gland epithelial cells, were used for this study (Schulze-Garg et al. 2000)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy Kit (Qiagen) was used according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 50 ng total RNA (GeneChip® Two-Cycle Target Labelling Kit (Affymetrix)).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix).
|
| Data processing |
GeneSpring GX 10.0 (Agilent) was used for data analysis.
|
| |
|
| Submission date |
Apr 21, 2010 |
| Last update date |
Apr 11, 2011 |
| Contact name |
Celine Schäfer |
| E-mail(s) |
celine.schaefer@charite.de
|
| Organization name |
Charité
|
| Department |
Med. Kl. m.S. Hepatologie und Gastroenterologie
|
| Lab |
AG Grötzinger
|
| Street address |
Augustenburger Platz 1
|
| City |
Berlin |
| ZIP/Postal code |
13353 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE21444 |
Expression profiling of murine DCIS and invasive ductal breast carcinoma |
|
