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| Status |
Public on Jul 15, 2021 |
| Title |
IR_d28 |
| Sample type |
SRA |
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| Source name |
Kidney
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| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: Kidney
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| Extracted molecule |
total RNA |
| Extraction protocol |
The kidney was homogenized using 21G and 26 1/2G syringes. Up to 0.25 g of the tissue was digested with 50ul of Enzyme D, 25 μl of Enzyme R and 6.75 μl of Enzyme A in 1 ml of RPMI and incubated for 30mins at 37°C. The reaction was deactivated by adding 10% FBS. The solution was then passed through a 40 μm cell strainer. After centrifugation at 1,000 RPM for 5 mins, cell pellet was incubated with 1 ml of RBC lysis buffer on ice for 3 mins. Cell number and viability were analyzed using Countess AutoCounter (Invitrogen, C10227).
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
none provided by the submitter
Genome_build: mm10
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| Submission date |
Jun 03, 2021 |
| Last update date |
Jul 17, 2021 |
| Contact name |
汉 朱 |
| E-mail(s) |
zhuhan19901007@126.com
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| Organization name |
肾
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| Street address |
1095 解放大道， 武汉 430030， 中国
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| City |
武汉 |
| State/province |
湖北 |
| ZIP/Postal code |
435000 |
| Country |
China |
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| Platform ID |
GPL24247 |
| Series (2) |
| GSE176097 |
The Probiotic L. casei Zhang Slows the Progression of Acute and Chronic Kidney Disease (scRNA-Seq) |
| GSE176098 |
The Probiotic L. casei Zhang Slows the Progression of Acute and Chronic Kidney Disease |
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| Relations |
| BioSample |
SAMN19549585 |
| SRA |
SRX11053903 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM5355893_IR_d28_barcodes.tsv.gz |
75.0 Kb |
(ftp)(http) |
TSV |
| GSM5355893_IR_d28_features.tsv.gz |
272.8 Kb |
(ftp)(http) |
TSV |
| GSM5355893_IR_d28_matrix.mtx.gz |
103.3 Mb |
(ftp)(http) |
MTX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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