|
| Status |
Public on May 29, 2024 |
| Title |
Xenograft tumor-Water-scRNA-seq |
| Sample type |
SRA |
| |
|
| Source name |
Xenograft tumor
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: xenograft-derived immune cells
treatment: water
|
| Treatment protocol |
gavage with DFMO or water twice a day for 14 days since 7-days after tumor incubation
|
| Growth protocol |
in SPF standard environment
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Single cells were suspended in calcium- and magnesium-free PBS containing 5% v/v FBS for further library construction
RNA libraries were prepared with Chromium Single cell 5'Library & Gel Bead according to the manufacturer's protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
The CellRanger(V3.02) was used to demultiplex the raw data and scanpy(v1.4.4) was utilized the following procedure to control for data quality: cells with fewer than 200 genes, and genes that are detected in less than 5 cells
Genome_build: mm10
Supplementary_files_format_and_content: Matrix tables with raw counts for each cell
|
| |
|
| Submission date |
May 30, 2021 |
| Last update date |
May 29, 2024 |
| Contact name |
Haoyan Chen |
| Organization name |
Renji Hospital
|
| Street address |
145 Shandong Road
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200001 |
| Country |
China |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE175805 |
Single cell sequencing to investigate the alteration of urea cycle in DFMO treated mouse |
|
| Relations |
| BioSample |
SAMN19459298 |
| SRA |
SRX11027930 |
