|
| Status |
Public on May 29, 2021 |
| Title |
miRNA Control, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Human Aortic Endothelial Cells
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: control
|
| Treatment protocol |
Cells were cultured to 80% confluence and then treated for 48h with 500μM L-Hcy to mimic severe HHcy seen in homocystinuria patients with CBS deficiencyvor with control medium.
|
| Growth protocol |
HAEC were grown in M199 medium (Thermo Scientific, Waltham, MA) containing 20% fetal calf serum (Thermo Scientific), 50μg/mL endothelial cell growth supplement (BD Biosciences, San Jose, CA), and 50μg/mL heparin. Cells from passage 6 were used for experiments.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocols
|
| |
|
| Hybridization protocol |
standard Affymetrix protocols
|
| Scan protocol |
standard Affymetrix protocols
|
| Data processing |
Pre-processing of microarray data was performed with Partek® Genomics Suite v6.5 (Partek Inc., St. Louis, MO) using a robust multi-array average (RMA) algorithm to interpret the raw signal.
|
| |
|
| Submission date |
May 28, 2021 |
| Last update date |
May 29, 2021 |
| Contact name |
Hong Wang |
| Organization name |
Temple University Lewis Katz School of Medicine
|
| Department |
CMDR
|
| Street address |
3500 N Broad St. MERB1060
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19140 |
| Country |
USA |
| |
|
| Platform ID |
GPL8786 |
| Series (2) |
| GSE175745 |
The effect of homocysteine on Human Aortic Endothelial Cells [miRNA] |
| GSE175748 |
The effect of homocysteine on Human Aortic Endothelial Cells |
|
