|
| Status |
Public on May 04, 2010 |
| Title |
C57Bl/6 E15 Male, hypothalamus and preoptic area |
| Sample type |
RNA |
| |
|
| Source name |
C57Bl/6 E15 Male, hypothalamus and preoptic area
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
sex: male
age: embryonic day 15
tissue: hypothalamus and preoptic area
|
| Biomaterial provider |
Charles River Labs.
|
| Treatment protocol |
Microdissection into RNAlater (Ambion).
|
| Growth protocol |
Standard chow, 12:12 light:dark cycle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Kit (QIAGEN).
|
| Label |
Biotin
|
| Label protocol |
One round amplification protocol for total RNA following Affymetrix’ specifications using T7 promoter oligo dT primer for cDNA synthesis, and ENZO IVT kit (www.affymetrix.com, GeneChip Expression Analysis, Technical Manual).
|
| |
|
| Hybridization protocol |
16hrs at 45° C with rotation (60rpm) as described by Affymetrix in their GeneChip Expression Analysis Technical Manual.
|
| Scan protocol |
Using Affymetrix’ GeneChip Scanner 3000 7G and default parameters described by the manufacturer in their GeneChip Expression Analysis Technical Manual.
|
| Description |
Hybridization was performed by the Johns Hopkins Medical Institutions Microarray Core Facility.
|
| Data processing |
Image analysis using GCOS 1.4, using MAS 5.0 algorithm and manufacturer’s specifications. Global scaling of images to a target intensity of 150.
|
| |
|
| Submission date |
Apr 08, 2010 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Seth Blackshaw |
| E-mail(s) |
sblack@jhmi.edu
|
| Organization name |
The Johns Hopkins University
|
| Department |
Neuroscience
|
| Street address |
733 N. Broadway, Suite 351
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE21278 |
A genomic atlas of mouse hypothalamic development |
|
| Relations |
| Reanalyzed by |
GSE119085 |
