|
| Status |
Public on Jun 24, 2011 |
| Title |
Macrophage control #1 treated with medium |
| Sample type |
RNA |
| |
|
| Source name |
Murine J774.A1 macrophages, without infection
|
| Organism |
Mus musculus |
| Characteristics |
treatment: without infection
cell line: Murine J774.A1 macrophages
|
| Treatment protocol |
J774.A1 macrophages were plated in T75 at 8 x 10^6 cells per flask one day. Total RNAs were isolated by TRIzol and further purified using Qiagen RNeasy Mini Kit (Qiagen, Valencia, CA). No infection was applied to these cells. This is replicate #1.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were isolated by TRIzol and further purified using Qiagen RNeasy Mini Kit (Qiagen, Valencia, CA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Total RNA (20 µg) per sample was used for hybridization with Affymetrix mouse GeneChip 430 2.0 array at the Core Laboratory Facility at the Virginia Bioinformatics Institute (VBI).
|
| Scan protocol |
Follow standard Affymetrix protocol.
|
| Description |
This is a macrophage sample without infection with B. suis rough strain VTRS1.
|
| Data processing |
The data was processed using RMA.
|
| |
|
| Submission date |
Mar 29, 2010 |
| Last update date |
Jun 24, 2011 |
| Contact name |
Yongqun He |
| E-mail(s) |
yongqunh@umich.edu
|
| Phone |
734-615-8231
|
| Fax |
734-936-3235
|
| URL |
http://www.hegroup.org/
|
| Organization name |
University of Michigan
|
| Street address |
018 ARF, 1150 W. Medical Center Dr.
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE21117 |
Microarrays of macrophages infected with smooth or rough Brucella suis |
|
