|
| Status |
Public on Jun 24, 2010 |
| Title |
Prostate tumor PCA0103 miRNA |
| Sample type |
RNA |
| |
|
| Source name |
human prostate
|
| Organism |
Homo sapiens |
| Characteristics |
sample id: PCA0103
tissue: Prostate
disease status: prostate cancer
tumor type: Primary tumor
biopsy_gleason_grade: 7
clint_stage: T1C
pathological_stage: T2B
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolaged using TRIzol@Reagent Total RNA Isoloation (Invitrogen), quantified with Thermo Scientific NanoDrop 1000 Spectrophotometer, and quality controlled with RNA 6000 picoAssay and Bioanalyzer 2100 (Agilent).
|
| Label |
Cy3
|
| Label protocol |
200ng of total RNA was labeled using the miRNA Complete Labeling and Hybridization Kit (Agilent)
|
| |
|
| Hybridization protocol |
Per manufacturer's instructions
|
| Scan protocol |
Per manufacturer's instructions
|
| Description |
microRNA expression profiling
|
| Data processing |
Images were quantified using Agilent Feature Extraction version 9.5 and data was normalized with between-array variance stabilization normalization (VSN) after excluding any microRNA not present in >80% of the samples profiled
|
| |
|
| Submission date |
Mar 24, 2010 |
| Last update date |
Jun 15, 2010 |
| Contact name |
Barry Stephen Taylor |
| Organization name |
Memorial Sloan-Kettering Cancer Center
|
| Street address |
1275 York Avenue, Box #460
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL8227 |
| Series (2) |
| GSE21032 |
Integrative genomic profiling of human prostate cancer |
| GSE21036 |
MicroRNA expression data for human primary and metastatic prostate cancer samples and control normal adjacent benign prostate |
|
