|
| Status |
Public on Jul 26, 2021 |
| Title |
BAT_control diet_rep1 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
MeDIP DNA from control diet
|
| Organism |
Mus musculus |
| Characteristics |
tissue: brown adipose tissue
age: 16 week
sample type: MeDIP DNA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from fat samples using Dneasy Blood & Tissue Kit.
|
| Label |
Cy5
|
| Label protocol |
Immunoprecipitated methylated DNA was labeled with Cy5 fluorophere and the input genomic DNA was labeled with Cy3 fluorophere.
|
| |
|
| Channel 2 |
| Source name |
input DNA from maternal control diet
|
| Organism |
Mus musculus |
| Characteristics |
tissue: brown adipose tissue
age: 16 week
sample type: input DNA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from fat samples using Dneasy Blood & Tissue Kit.
|
| Label |
Cy3
|
| Label protocol |
Immunoprecipitated methylated DNA was labeled with Cy5 fluorophere and the input genomic DNA was labeled with Cy3 fluorophere.
|
| |
|
| |
| Hybridization protocol |
Labeled DNA was hybridized to the Arraystar Mouse ReqSeq Promoter Array.
|
| Scan protocol |
Array was scanned with Agilent Scanner G2505C.
|
| Description |
maternal control diet
|
| Data processing |
After normalization, raw data was input into SignalMap Software to observe and evaluate the methylation peaks.
|
| |
|
| Submission date |
Apr 23, 2021 |
| Last update date |
Jul 27, 2021 |
| Contact name |
Qian Zhang |
| Organization name |
Peking Union Medical College Hospital
|
| Street address |
No. 1 Shuaifuyuan, Dongcheng District
|
| City |
Beijing |
| ZIP/Postal code |
100730 |
| Country |
China |
| |
|
| Platform ID |
GPL21852 |
| Series (1) |
| GSE173218 |
DNA methylation alternations in the offspring brown adipose tissue from high fat diet dams |
|
