|
| Status |
Public on Jun 24, 2010 |
| Title |
Prostate tumor PCA0025 exon |
| Sample type |
RNA |
| |
|
| Source name |
human prostate
|
| Organism |
Homo sapiens |
| Characteristics |
sample id: PCA0025
tissue: Prostate
disease status: prostate cancer
tumor type: Primary tumor
biopsy_gleason_grade: 7
clint_stage: T2B
pathological_stage: T2C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolaged using TRIzol@Reagent Total RNA Isoloation (Invitrogen), quantified with Thermo Scientific NanoDrop 1000 Spectrophotometer, and quality controlled with RNA 6000 picoAssay and Bioanalyzer 2100 (Agilent).
|
| Label |
biotin
|
| Label protocol |
Per manufacturer's instructions with the Affymetrix WT Sense Target Labeling Assay
|
| |
|
| Hybridization protocol |
Per manufacturer's instructions
|
| Scan protocol |
Affymetrix high-numeric aperture and flying objective lens (FOL) GS3000 scanner
|
| Description |
exon and whole-transcript expression profiling
|
| Data processing |
Images were quantified using the GeneChip Operating Software (GCOS) version 1.4. Data were processed with Aroma Affymetrix using standard RMA background adjustment and quantile normalization
|
| |
|
| Submission date |
Mar 24, 2010 |
| Last update date |
Jun 15, 2010 |
| Contact name |
Barry Stephen Taylor |
| Organization name |
Memorial Sloan-Kettering Cancer Center
|
| Street address |
1275 York Avenue, Box #460
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL5188 |
| Series (2) |
| GSE21032 |
Integrative genomic profiling of human prostate cancer |
| GSE21034 |
Whole-transcript and exon-level expression data for human primary and metastatic prostate cancer samples and control normal adjacent benign prostate |
|
| Relations |
| Alternative to |
GSM527877 (exon-level analysis) |
