|
| Status |
Public on Dec 31, 2011 |
| Title |
(745)Morphology:Brachial artery of pig#9481 |
| Sample type |
RNA |
| |
|
| Source name |
Brachial artery of pig#9481
|
| Organism |
Sus scrofa |
| Characteristics |
artery: Brachial artery
pig id: 9481
cell type: blood, whole
age: 1 week
|
| Growth protocol |
Pigs were on a normal diet
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Macherey Nagel Nucleo Spin II for the piglets and CsCl ultracentrifugation followed by MN Nucleospin II for the one and two year olds.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared by the GeneChip One-Cycle target labeling kit according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 6.5 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Porcine Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 (affymetrix).
|
| Description |
Brachial artery of pig#9481
|
| Data processing |
Default GCOS 1.4 raw probe intensities were analyzed using the mas5 module of the affy 1.0 package for R version 2.3.1. The probesets that were present according to MAS5calls and normalized, background corrected, and summarized using the RMA function in Bionconductor.
|
| |
|
| Submission date |
Mar 24, 2010 |
| Last update date |
Dec 31, 2011 |
| Contact name |
Martin Bahls |
| E-mail(s) |
mbahls@purdue.edu
|
| Organization name |
Purdue University
|
| Street address |
800 W Stadium Ave
|
| City |
West Lafayette |
| ZIP/Postal code |
47906 |
| Country |
USA |
| |
|
| Platform ID |
GPL3533 |
| Series (1) |
| GSE21043 |
Gene expression differences between the brachial and femoral artery of the Rapacz HC swine |
|
