|
| Status |
Public on Sep 01, 2010 |
| Title |
DRG_DR_72h_rep1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
L4, L5 and L6 dorsal root ganglia
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: dorsal root ganglia
time: 72h after dorsal root crush
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
Agilent labeling kit, according to manufacturers instructions.
|
| |
|
| Channel 2 |
| Source name |
L4, L5 and L6 dorsal root ganglia
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: unlesioned dorsal root ganglia pooled from 3 animals
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Agilent labeling kit, according to manufacturers instructions.
|
| |
|
| |
| Hybridization protocol |
According to manufacturers instructions.
|
| Scan protocol |
Agilent scanner
|
| Description |
gene expression data of regenerating dorsal root ganglion neurons
|
| Data processing |
The data were analyzed with the R packages 'bioconductor' and 'limma'. Raw spot intensities were background corrected using Edwards method, loess normalized within arrays and normalized between arrays using Aquantile method.
|
| |
|
| Submission date |
Mar 22, 2010 |
| Last update date |
May 27, 2010 |
| Contact name |
Harold D MacGillavry |
| E-mail(s) |
hmacgillavry@gmail.com
|
| Organization name |
Vrije Universiteit
|
| Department |
Center for neurogenomics and cognitive research
|
| Lab |
Molecular and cellular neurobiology
|
| Street address |
De Boelelaan 1087
|
| City |
Amsterdam |
| ZIP/Postal code |
1081HV |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL7289 |
| Series (1) |
| GSE21007 |
Whole-genome gene expression profiling of dorsal root ganglion neurons after nerve damage |
|
