|
| Status |
Public on May 01, 2021 |
| Title |
SAMPLE 10_MDAPCa2b |
| Sample type |
RNA |
| |
|
| Source name |
MDAPCa2b cell line, grown under normal conditions
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDAPCa2b
passage number: 31
cell type: Prostate epithelium
Stage: Bone Metastatic
racial background: African American
|
| Treatment protocol |
None
|
| Growth protocol |
LNCaP: ATCC RPMI, 10%FBS, 100 ug/ml penicillin-streptomycin.
22RV1: ATCC RPMI, 10%FBS, 100 ug/ml penicillin-streptomycin.
DU145: DMEM, 10%FBS, 100 ug/ml penicillin-streptomycin.
PC3: ATCC RPMI, 10%FBS, 100 ug/ml penicillin-streptomycin.
VCaP: DMEM-F12 Ham 1:1, 10%FBS, 100 ug/ml penicillin-streptomycin.
MDAPCa2a: HPC1 Media, 10%FBS, 100 ug/ml penicillin-streptomycin. FNC coating Mix (Athena Enzyme Systems)
MDAPCa2b: HPC1 Media, 10%FBS, 100 ug/ml penicillin-streptomycin. FNC coating Mix (Athena Enzyme Systems)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy with DNAse was used on 4 million cell pellets, followed by tratement with RNeasy MinElute Cleanup Kit with DNAse treatment as per the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Carried out by Thermo Fisher Scientific Microarray Analysis Services
|
| |
|
| Hybridization protocol |
Carried out by Thermo Fisher Scientific Microarray Analysis Services
|
| Scan protocol |
Carried out by Thermo Fisher Scientific Microarray Analysis Services
|
| Description |
Gene expression data from cells grown under normal condtions
|
| Data processing |
Carried out by Thermo Fisher Scientific Microarray Analysis Services: Summarization SST-RMA algorithm1 was carried out in Transcriptome Analysis Console (TAC) Software
|
| |
|
| Submission date |
Apr 14, 2021 |
| Last update date |
May 02, 2021 |
| Contact name |
Rebeca SanMartin |
| E-mail(s) |
rsanmart@utk.edu
|
| Organization name |
University of Tennessee Knoxville
|
| Street address |
1311 Cumberland Avenue
|
| City |
Knoxville |
| State/province |
TN |
| ZIP/Postal code |
37996 |
| Country |
USA |
| |
|
| Platform ID |
GPL23159 |
| Series (2) |
| GSE172098 |
Alterations in chromosome spatial compartmentalization classify prostate cancer progression [array] |
| GSE172099 |
Alterations in chromosome spatial compartmentalization classify prostate cancer progression. |
|
