|
| Status |
Public on Feb 23, 2023 |
| Title |
EphA2_T0 |
| Sample type |
genomic |
| |
|
| Source name |
Breast Cancer ex vivo cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-MB-231
|
| Treatment protocol |
ex vivo cell were treated with IgG or anti-EphA2 as described in the manuscript Zawily et al.
|
| Growth protocol |
ex vivo cells were collected as describled in the manuscript Zawily et al.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was extracted as described in Blakely et al. Methods in Molecular Biology. 2011 PMID: 2187728
|
| Label |
T0 Timepoint
|
| Label protocol |
labeling was done as described in Blakely et al. Methods in Molecular Biology. 2011 PMID: 2187728
|
| |
|
| Hybridization protocol |
hybing was done as described in Blakely et al. Methods in Molecular Biology. 2011 PMID: 2187728
|
| Scan protocol |
scanning was done as described in Blakely et al. Methods in Molecular Biology. 2011 PMID: 2187728
|
| Description |
Anti-EphA2 Treated
|
| Data processing |
The raw intensity data extracted from the CEL files were normalized with cyclic loess normalization and log transformed. This data was later used to calculate the drop in expression between and initial and final time points for each replicates. The details of differential expression calculations are explained in the manuscript.
|
| |
|
| Submission date |
Apr 12, 2021 |
| Last update date |
Feb 23, 2023 |
| Contact name |
Franco J Vizeacoumar |
| E-mail(s) |
franco.vizeacoumar@usask.ca
|
| Phone |
306-966-7010
|
| Organization name |
University of Saskatchewan
|
| Department |
Oncology
|
| Lab |
Vizeacoumar Lab
|
| Street address |
4D40 Health Sciences Building, 107 Wiggins Road
|
| City |
Saskatoon |
| State/province |
Saskatchewan |
| ZIP/Postal code |
S7N 5E5 |
| Country |
Canada |
| |
|
| Platform ID |
GPL29998 |
| Series (1) |
| GSE171920 |
A Multipronged Unbiased Strategy Guides the Development of an anti-EGFR/EPHA2 Bispecific Antibody for Combination Cancer Therapy |
|
