|
| Status |
Public on Mar 27, 2024 |
| Title |
T24-1 |
| Sample type |
SRA |
| |
|
| Source name |
T24
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: T24
genotype: control
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was extracted using Trizol reagent according to the manufacturer’s instructions.
The construction of RNA-seq library was based on the protocol of the IlluminaTruSeq RNA Sample Preparation Kit (illumina)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to human genome (hg38) using the CLC Biomedical Genomics Workbench 4 (QIAGEN Bioinformatics).
The read numbers mapped of each gene was counted and Reads Per Kilobase of exon model per Million mapped reads,(RPKM) of each gene was calculated based on the length of the gene and reads count mapped to this gene by using the CLC Biomedical Genomics Workbench 4 (QIAGEN Bioinformatics).
Genome_build: hg38
Supplementary_files_format_and_content: Tab-delimited text files include RPKM values for each Sample.
|
| |
|
| Submission date |
Mar 29, 2021 |
| Last update date |
Mar 27, 2024 |
| Contact name |
Zengnan Mo |
| E-mail(s) |
mozengnan@gxmu.edu.cn
|
| Phone |
15188577508
|
| Organization name |
Guangxi Medical University
|
| Department |
Center for Genomics and Personalized Medicine
|
| Street address |
Zhongshan Street
|
| City |
Nanning |
| State/province |
Guangxi |
| ZIP/Postal code |
530021 |
| Country |
China |
| |
|
| Platform ID |
GPL24676 |
| Series (2) |
| GSE171022 |
Potential Roles of IGF2BP3 in Bladder Cancer [RNA-Seq] |
| GSE171024 |
Potential Roles of IGF2BP3 in Bladder Cancer |
|
| Relations |
| BioSample |
SAMN18524277 |
| SRA |
SRX10465363 |
