|
| Status |
Public on Jan 11, 2022 |
| Title |
4U8C-KO_2 |
| Sample type |
SRA |
| |
|
| Source name |
BMDC
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: Bat3 fl/fl CD11c Cre
treatment: 4µ8C
|
| Treatment protocol |
Five biological replicates with treatments for 8 hours.
|
| Growth protocol |
Bone marrow derive dendritic cells were routinely generated by re-plating d9 bone marrow cell differentiation cultures (typically 2–3 × 106 cells into 100mm diameter dish) on uncoated plastic in complete RPMI1640 (Gibco) supplemented with 20 ng/ml of GM-CSF (PeproTech).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
BMDCs were harvest, live CD11c hi BMDCs were sorted into lysis buffer, flash frozen on dry ice. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
SK-UG7_F12
|
| Data processing |
Bcl2Fastq
RSEM Gene quantification
|
| |
|
| Submission date |
Mar 24, 2021 |
| Last update date |
Jan 11, 2022 |
| Contact name |
Ayshwarya Subramanian |
| E-mail(s) |
subraman@broadinstitute.org
|
| Organization name |
Broad Institute
|
| Department |
Klarman Cell Observatory
|
| Street address |
415 Main Street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL9250 |
| Series (2) |
| GSE169550 |
Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Bat3-/- BMDC Transcriptomes II |
| GSE169664 |
Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Bat3-/- BMDC Transcriptomes |
|
| Relations |
| BioSample |
SAMN18474654 |
| SRA |
SRX10437306 |
