|
| Status |
Public on Jan 31, 2022 |
| Title |
Main olfactory bulb replicate 1 slide 1 |
| Sample type |
SRA |
| |
|
| Source name |
Main olfactory bulb
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Main olfactory bulb
genotype: wild type
gender: male
|
| Treatment protocol |
Male mice were euthanized according to our IACUC protocol. The anterior part of the brain containing the olfactory bulb was dissected and placed in a plastic mold filled with OCT (Sakura Finetek from VWR), The molds were then frozen with dry ice pre-chilled 2-methylbutane (Fisher Scientific). The samples were incubated in -80°C overnight prior to cryosectioning. 20 OB sections were obtained with equal spacing (130 μm) along the anterior-posterior axis. In total, two sets of 20 slides were obtained from different animals.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
The extraction and library protocol were performed as previously described (PMID: 33288904)
|
| |
|
| Library strategy |
OTHER |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
OB1_slide1
|
| Data processing |
Library strategy: Slide-seq V2
The data processing were performed as previously described (PMID: 33288904)
All the downstring analysis were processed with Seurat, the scripts can be found at https://github.com/Greerlab/OSN_2021_paper
Genome_build: mm10
Supplementary_files_format_and_content: Seruat object containing the raw and normalized count, PCA, UMAP, spatial coordinates
|
| |
|
| Submission date |
Mar 16, 2021 |
| Last update date |
Jan 31, 2022 |
| Contact name |
I-Hao Wang |
| Organization name |
UMMS
|
| Department |
PMM
|
| Street address |
370 Sunderland Rd, Unit D24
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01604 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (2) |
| GSE169012 |
Spatial transcriptome analysis (Slide-seq2) of mouse main olfactory bulb |
| GSE169021 |
Mouse olfactory sensory neurons, main olfactory epithelium and main olfactory bulb |
|
| Relations |
| BioSample |
SAMN18320385 |
| SRA |
SRX10355485 |
