|
| Status |
Public on Aug 05, 2021 |
| Title |
retina, DM |
| Sample type |
SRA |
| |
|
| Source name |
retina
|
| Organism |
Macaca fascicularis |
| Characteristics |
disease state: diabetes mellitus
tissue: retina
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
All retina tissues were digested at 37℃, and were dissociated and triturated into single cell suspensions with 0.04% bovine serum albumin (BSA) in PBS solution. Single cell suspensions were diluted at a concentration of 500-1800 cells/uL in 0.04% BSA/Ames.
10x Genomics Chromium single cell system with v2
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
Cellranger v3.1 was used for demultiplexing, aligment and quantification.
Genome_build: Macaca_fascicularis_5.0
Supplementary_files_format_and_content: h5 file
|
| |
|
| Submission date |
Mar 15, 2021 |
| Last update date |
Aug 05, 2021 |
| Contact name |
yuhua xiao |
| E-mail(s) |
xiaoyh26@mail2.sysu.edu.cn
|
| Phone |
18728192414
|
| Organization name |
Sun Yat-sen University
|
| Department |
zhongshan Ophthalmic center
|
| Street address |
jinsui load
|
| City |
guangzhou |
| State/province |
guangdong province |
| ZIP/Postal code |
510627 |
| Country |
China |
| |
|
| Platform ID |
GPL23096 |
| Series (1) |
| GSE168908 |
Single-cell transcriptome profiling reveals the suppressive role of retinal neurons in microglia activation under diabetes mellitus |
|
| Relations |
| BioSample |
SAMN18311288 |
| SRA |
SRX10341670 |
