|
| Status |
Public on Mar 11, 2024 |
| Title |
Control rep2 |
| Sample type |
SRA |
| |
|
| Source name |
Placenta from control mice
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: Placenta
devolopmental stage: Placenta at pregnant 17.5 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Placetnas on pregnant 17.5 days were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
mRNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
Control-rep2
|
| Data processing |
Reads filtering under criteria removing reads with 20% of the base quality lower than 13
HISAT2.1.0 mapping to reference genome
count counting
Genome_build: GRCm38
Supplementary_files_format_and_content: TXT file contain counts for each sample
|
| |
|
| Submission date |
Mar 11, 2021 |
| Last update date |
Mar 11, 2024 |
| Contact name |
Wen-Long Zhao |
| E-mail(s) |
zbyzwl1919@sina.com
|
| Organization name |
The International Peace Maternity and Child Health Hospital
|
| Street address |
Guangyuan road 145#
|
| City |
Shanghai |
| ZIP/Postal code |
200030 |
| Country |
China |
| |
|
| Platform ID |
GPL21273 |
| Series (1) |
| GSE168702 |
Next Generation Sequencing Facilitates Quantitative Analysis of mRNA expression prolifes in placentas from wild Type,early-onset and later-onset PE mouse models |
|
| Relations |
| BioSample |
SAMN18256837 |
| SRA |
SRX10312957 |
