|
| Status |
Public on Aug 06, 2010 |
| Title |
NCI-H1693 [50K_Xba] |
| Sample type |
genomic |
| |
|
| Source name |
NSCLC Cell Line NCI-H1693
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: NSCLC
cell line: NSCLC Cell Line NCI-H1693
|
| Growth protocol |
Cell lines were grown according to standard conditions.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
As per manufacturer (Affymetrix)
|
| Label |
Biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000 G7.
|
| Description |
Hybridized to Xba240
|
| Data processing |
Feature data were collected and extracted from the scanner using the Affymetrix GeneChip® Operating Software (GCOS). Affymetrix 100K Mapping Array intensity signal CEL files were processed by Partek software (Build 6.08.0623) using the PM/MM difference model. Ninety normal samples were previously downloaded from the Affymetrix website (http://www.affymetrix.com/support/technical/byproduct.affx?product=100k) and averaged to establish the reference for calculating the copy numbers.
|
| |
|
| Submission date |
Feb 23, 2010 |
| Last update date |
Apr 29, 2011 |
| Contact name |
Xin Lu |
| E-mail(s) |
xin.x.lu@abbott.com
|
| Phone |
847-937-7942
|
| Organization name |
Abbott Laboratories
|
| Department |
Cancer Research
|
| Lab |
Cancer Genomics
|
| Street address |
100 Abbott Park Rd
|
| City |
Abbott Park |
| State/province |
IL |
| ZIP/Postal code |
60064 |
| Country |
USA |
| |
|
| Platform ID |
GPL2005 |
| Series (1) |
| GSE20481 |
Affymetrix SNP array data for NSCLC and CRC |
|
