|
| Status |
Public on Mar 03, 2023 |
| Title |
C13_R2 RNA |
| Sample type |
SRA |
| |
|
| Source name |
KIT+ CD16/32- CD34dim CD41- CD9-
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: Primary bone marrow
genotype: Wild type
cell population: KIT+ CD16/32- CD34dim CD41- CD9-
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from primary mouse bone marrow using the Ambion PureLink RNA Mini Kit (Thermo Fisher cat# 9655) with Trizol according to the maufacturer's protocol.
Libraries were constructed from 5 ng total RNA using the Ovation RNA-Seq System V2 (Tecan). RNA-Seq libraries were prepared from 1 ug of cDNA using the TruSeq Stranded RNA Kit. Due to the amplification method, libraries are not stranded. Libraries were sequenced on the NovaSeq 6000.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Raw sequencing data was processed with RTA (v1.18.54.4).
Alignment was performed using STAR (v2.7.2a)
Transcript expression was estimated using RSEM (v1.3.2) and edgeR (v3.30.3)
Genome_build: mm10
Supplementary_files_format_and_content: TPM counts
|
| |
|
| Submission date |
Mar 04, 2021 |
| Last update date |
Nov 20, 2024 |
| Contact name |
Jens Lichtenberg |
| E-mail(s) |
lichtenbergj@mail.nih.gov
|
| Phone |
7402749495
|
| Fax |
3014024929
|
| Organization name |
National Institutes of Health
|
| Department |
National Human Genome Research Institute
|
| Street address |
49 Convent Dr 3A19
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (2) |
| GSE168258 |
Identification and isolation of hematopoietic stem and progenitor cell populations (RNA-seq) |
| GSE168260 |
Identification and isolation of hematopoietic stem and progenitor cell populations |
|
| Relations |
| BioSample |
SAMN18142673 |
| SRA |
SRX10240660 |
