|
| Status |
Public on Sep 29, 2021 |
| Title |
P55: European placenta mRNA |
| Sample type |
RNA |
| |
|
| Source name |
placenta
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
tissue: Placenta
population: European
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from placentas was isolated using RNeasy Mini Kits according to the manufacturer’s instructions. RNA quantity was determined using an Agilent Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
cRNA in hybridization solution was denatured at 99℃ for 5 min and 45℃ for 5 min prior to loading onto a microarray. Arrays were hybridized in a Agilent Hybridization Oven for 16hr at a rotation speed of 60 rpm at 45℃ and washed with Wash Buffer A and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Scanned on Affymetrix GeneChip Scanner 3000
|
| Description |
Gene expression data from European placenta
|
| Data processing |
AGCC Software (Affymetrix® GeneChip® Command Console® Software) converts.DAT files from image signals to digital signals known as.CEL files. CEL files record probe level fluorescent signals.
|
| |
|
| Submission date |
Mar 02, 2021 |
| Last update date |
Sep 29, 2021 |
| Contact name |
Tanna Wuren |
| E-mail(s) |
tanna.mgl@gmail.com
|
| Organization name |
Qinghai University
|
| Street address |
Kunlun Road No.16
|
| City |
xining |
| ZIP/Postal code |
810001 |
| Country |
China |
| |
|
| Platform ID |
GPL15207 |
| Series (2) |
| GSE168021 |
Gene expression in Placental Tissue from high altitude European and Tibetan women |
| GSE168130 |
miRNA and mRNA expression in placental tissue from high altitude pregnant women |
|
