|
| Status |
Public on Feb 19, 2021 |
| Title |
AS57542-Granulocytes |
| Sample type |
genomic |
| |
|
| Source name |
Granulocytes
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: F
patientid: AS57542
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from frozen whole blood samples using a standard cell lysis, proteinase K digestion, phenol/chloroform, ethanol precipitation method.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
500 ng DNA from each sample was sodium bisulfite-treated using the EZ-96 DNA Methylation-Gol Kit (Zymo Research) according to the manufacturerÔÇÖs standard protocol.
|
| |
|
| Hybridization protocol |
Samples were processed according to the Illumina Infinium HumanMethylationEPIC BeadChip (Illumina) protocol with the exception that 7ul of bisulfite treated material was input into the whole genome amplicifaction reaction instead of 4ul.
|
| Scan protocol |
amples were assessed using the Illumina Infinium HumanMethylationEPIC BeadChip (Illumina) using a Illumina iScan System (Illumina).
|
| Description |
200861170109_R04C01
|
| Data processing |
Data was imported using the R package methylumi methylumIDAT function. Data was then pre-processed in the R package wateRmelon using the pfilter and dasen functions. Processed data file includes average Beta values.
Normalised (dasen) Average Beta
Unmethylated and methylated signal intensities
|
| |
|
| Submission date |
Feb 16, 2021 |
| Last update date |
Mar 08, 2021 |
| Contact name |
Emma Walker |
| E-mail(s) |
e.m.walker@exeter.ac.uk
|
| Organization name |
University of Exeter
|
| Street address |
RILD Barrack Rd
|
| City |
Exeter |
| ZIP/Postal code |
EX2 5DW |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL21145 |
| Series (1) |
| GSE166844 |
DNA methylation profiles of Purified Blood Cell types |
|
