|
| Status |
Public on Mar 12, 2010 |
| Title |
MCF-10A cells, shRNA vector control, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
MCF-10A cells, shRNA vector control
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF-10A
|
| Treatment protocol |
Retroviral or adenoviral infection for 48 hours
|
| Growth protocol |
Standard MCF10A growth conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy miniprep columns (Qiagen) were used according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNAs were prepared according to the Affymetrix standard labeling protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug cRNA were hybridized to the Affymetrix GeneChip Human Genome U133Av2 for 16 hrs. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneArray Chip Scanner, HP/Affymetrix Inc.
|
| Description |
shRNA_vector2
|
| Data processing |
Background correction and normalization of microarray data was performed using the Robust Multichip Average method implemented in the rma function of R/BioConductor.
|
| |
|
| Submission date |
Feb 11, 2010 |
| Last update date |
Mar 11, 2010 |
| Contact name |
Joan S. Brugge |
| E-mail(s) |
joan_brugge@hms.harvard.edu
|
| Organization name |
Harvard Medical School
|
| Department |
Department of Cell Biology
|
| Lab |
Brugge
|
| Street address |
240 Longwood Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE20286 |
Gene expression profiles induced by knockdown and overexpression of p63 variants in MCF-10A mammary epithelial cell line |
|
