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| Status |
Public on Feb 05, 2021 |
| Title |
Y_WT_618 |
| Sample type |
SRA |
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| Source name |
myeloid biased HSC
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| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: myeloid biased HSC
genotype: Igf2bp2 WT
age: 3 Months
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by using MagMax96 total RNA isolation kit (Ambion, AM1830) according to the manufacturer’s protocol.
RNA libraries were prepared for sequencing using Nextera XT DNA Library Preparation Kit
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
Total RNA sequenced reads were pseudoaligned to the mm10 reference cDNA using Salmon v1.4.0.
Single Cell RNA sequenced reads were aligned to the mm10 reference genome using 10X Genomics Cell Ranger v5.0.0.
Genome_build: mm10
Supplementary_files_format_and_content: Tar files with the output of Salmon, including quant.sf containing the TPM values
Supplementary_files_format_and_content: Tar files with raw and filtered gene matrices, features, and barcodes
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| Submission date |
Feb 04, 2021 |
| Last update date |
Feb 05, 2021 |
| Contact name |
Pura Muñoz-Cánoves |
| Organization name |
Altos Labs
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| Lab |
Muñoz-Cánoves
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| Street address |
5510 Morehouse Dr
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| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92121 |
| Country |
USA |
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| Platform ID |
GPL24247 |
| Series (1) |
| GSE166176 |
Bulk and single RNA sequencing on the role of Igf2bp2 in myeloid-biased HSC |
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| Relations |
| BioSample |
SAMN17796865 |
| SRA |
SRX10024536 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM5065194_21_Y_WT_618_S14.tar.gz |
1.9 Mb |
(ftp)(http) |
TAR |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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