|
| Status |
Public on Feb 03, 2021 |
| Title |
02_fiblo |
| Sample type |
SRA |
| |
|
| Source name |
E01F fibroblast
|
| Organism |
Callithrix jacchus |
| Characteristics |
cell type: Fibroblast
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were sorted into 96 well plate with a flow cytometer SH800 (SONY) and dissolved with single cell lysis buffer
Library were constructed using GenNext RamDA-seqTM Single Cell Kit and Netera XT library prep kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Sequenced reads were trimmed using trimmomatic based on reads quality and adaptor sequence
The resulting reads were mapped to cj1700 using STAR version 2.6.0c
Reads mapped to exon regions were counted using featureCount version 1.5.1.
Raw count data was loaded to R software and normalized using Seurat version 3.0.0.
Genome_build: cj1700
Supplementary_files_format_and_content: tab-delimited text files include normalized values for each Sample.
|
| |
|
| Submission date |
Feb 03, 2021 |
| Last update date |
Feb 04, 2021 |
| Contact name |
Sho Yoshimatsu |
| E-mail(s) |
yoshima@a7.keio.jp
|
| Organization name |
Keio University School of Medicine
|
| Department |
Physiology
|
| Lab |
Hideyuki Okano's Lab
|
| Street address |
35 Shinanomachi
|
| City |
Shinjuku |
| State/province |
Tokyo |
| ZIP/Postal code |
160-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17712 |
| Series (2) |
| GSE152493 |
Non-viral Induction of Transgene-free iPSCs from Somatic Fibroblasts of Multiple Mammalian Species |
| GSE166076 |
Non-viral Induction of Transgene-free iPSCs from Somatic Fibroblasts of Multiple Mammalian Species [marmoset_single-cell] |
|
| Relations |
| BioSample |
SAMN17768675 |
| SRA |
SRX10012117 |
