NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM505804 Query DataSets for GSM505804
Status Public on Feb 03, 2010
Title WSB/EiJ_Male_Nuc_Acc
Sample type RNA
 
Source name pooled tissue, Nucleus Accumbens, 8-10 week old, male, (n=3)
Organism Mus musculus
Characteristics gender: male
tissue: Nucleus Accumbens
strain: WSB/EiJ
Growth protocol Eight to ten week old male mice of each strain were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). The mice were habituated for one week prior to tissue collection. Animals were housed in an SPF barrier colony. Animals were maintained on a 12-h light: 12-h dark cycle, housed in groups of 2Ð4 in standard high efficiency particulate air-filtered polycarbonate mouse cages containing a layer of Bed-o-cob bedding and one cotton nestlet. Food (Pico rodent chow 20; Purina, St Louis, MO, USA) and water were made available ad libitum.
Extracted molecule total RNA
Extraction protocol After dissection, tissues were immediately frozen on dry ice and stored in a -80 freezer until RNA preparation. Tissues were pulverized to a fine powder while frozen to obtain a mixture of homogeneous tissue. A small (~10mg) aliquot was homogenized using a rotor-stator (Omni TH polytron) homogenizer in Trizol (Invitrogen) and total RNA purified with RNeasy columns (Qiagen).
Label biotin
Label protocol RNA was amplified and labeled using the Affymetrix one-cycle target labeling kit.
 
Hybridization protocol cDNA synthesis and amplification employed the Affymetrix 3' IVT protocol
Scan protocol standard Affymetrix procedures using the GeneChip 3000 scanner
Description n/a
Data processing data were processed using the gcRMA algorithm, as described in Wu. Z et al. 2004
 
Submission date Feb 02, 2010
Last update date Feb 03, 2010
Contact name Samantha Katherine Segall
Organization name UNC- Chapel Hill
Lab Wiltshire Lab
Street address 120 Mason Farm Road
City Chapel Hill
State/province NC
ZIP/Postal code 27599
Country USA
 
Platform ID GPL1261
Series (1)
GSE20160 Gene Expression Data from Prefrontal Cortex and Nucleus Accumbens from Inbred Strains of Mice

Data table header descriptions
ID_REF
VALUE gcRMA signal intensity

Data table
ID_REF VALUE
1415670_at 511.291
1415671_at 957.275
1415672_at 721.177
1415673_at 91.757
1415674_a_at 234.107
1415675_at 145.936
1415676_a_at 588.559
1415677_at 328.618
1415678_at 392.198
1415679_at 553.006
1415680_at 30.823
1415681_at 333.144
1415682_at 125.001
1415683_at 218.74
1415684_at 93.283
1415685_at 129.62
1415686_at 640.343
1415687_a_at 4069.539
1415688_at 349.52
1415689_s_at 133.624

Total number of rows: 45015

Table truncated, full table size 791 Kbytes.




Supplementary file Size Download File type/resource
GSM505804.CEL.gz 5.8 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap