|
| Status |
Public on Feb 03, 2010 |
| Title |
CE/J_Male_Frontal_Cortex |
| Sample type |
RNA |
| |
|
| Source name |
pooled tissue, Prefrontal Cortex, 8-10 week old, male, (n=3)
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
tissue: Frontal Cortex
strain: CE/J
|
| Growth protocol |
Eight to ten week old male mice of each strain were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). The mice were habituated for one week prior to tissue collection. Animals were housed in an SPF barrier colony. Animals were maintained on a 12-h light: 12-h dark cycle, housed in groups of 2Ð4 in standard high efficiency particulate air-filtered polycarbonate mouse cages containing a layer of Bed-o-cob bedding and one cotton nestlet. Food (Pico rodent chow 20; Purina, St Louis, MO, USA) and water were made available ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After dissection, tissues were immediately frozen on dry ice and stored in a -80 freezer until RNA preparation. Tissues were pulverized to a fine powder while frozen to obtain a mixture of homogeneous tissue. A small (~10mg) aliquot was homogenized using a rotor-stator (Omni TH polytron) homogenizer in Trizol (Invitrogen) and total RNA purified with RNeasy columns (Qiagen).
|
| Label |
biotin
|
| Label protocol |
RNA was amplified and labeled using the Affymetrix one-cycle target labeling kit.
|
| |
|
| Hybridization protocol |
cDNA synthesis and amplification employed the Affymetrix 3' IVT protocol
|
| Scan protocol |
standard Affymetrix procedures using the GeneChip 3000 scanner
|
| Description |
n/a
|
| Data processing |
data were processed using the gcRMA algorithm, as described in Wu. Z et al. 2004
|
| |
|
| Submission date |
Feb 02, 2010 |
| Last update date |
Feb 03, 2010 |
| Contact name |
Samantha Katherine Segall |
| Organization name |
UNC- Chapel Hill
|
| Lab |
Wiltshire Lab
|
| Street address |
120 Mason Farm Road
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE20160 |
Gene Expression Data from Prefrontal Cortex and Nucleus Accumbens from Inbred Strains of Mice |
|
