cell type: B cells cell line: CH12.LX array type: Mouse430_2 amount to core: 10 units of amount: ug od260: 2.198 od280: 1.084 ratio: 2.03 sample preservation: -20 in Nuclease free dH2O (RNeasy kit)
Biomaterial provider
Nadira De Abrew, CIIT Centers for Health Research, 6 Davis Drive, Research Triangle Park, NC, 27709, Nadira , ndeabrew@thehamner.org, 9195581388, 9195581300
Treatment protocol
in vitro Treatment: Untreated, Treatment Duration: 0, Units of Duration: hr, Vehicle for Chemical: DMSO, Route of Exposure: media, Dose or Concentration: 0, Units of Dose or Concentration: nM
Growth protocol
Growth Media: RPMISAdvance, HIFBS, HEPES, Pen/Strepp, L Glutamine, Mercaptoethanol, Amount of Media: 3, Units of Media: ml
Extracted molecule
total RNA
Extraction protocol
Extraction Method: RNeasy Additional Purification Method: Concentrated on Speed Vac
Label
biotin
Label protocol
standard Affymetrix protocol
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Sample Type: B cell line, Number of Cells Seeded: 1X10^5/ml, 3ml W of 6W plate, 4 or 2 wells per treatment depending on timepoint, Cell Type: CH12. LX, Harvest Time Point: 0, Units of Harvest Time Point: hr
Time Course Gene Expression Microarray Analysis of a Mouse B-cell Line (CH12.LX) Activated with Lipopolysaccharide and Treated with 2,3,7,8-Tetrachlorodibenzo-p-dioxin
Data table header descriptions
ID_REF
VALUE
Signal
ABS_CALL
indicating whether the transcript was present (P), absent (A), or marginal (M)
