|
Status |
Public on Dec 19, 2020 |
Title |
deep senescent cells - H3K27me3_rep1 |
Sample type |
SRA |
|
|
Source name |
foreskin fibroblast
|
Organism |
Homo sapiens |
Characteristics |
cell line: BJ treatment: bleomycin (40 ug/ml; Biorbyt) for 2 hours and culture for 28 days cell state: DS chip antibody: H3K27me3
|
Extracted molecule |
genomic DNA |
Extraction protocol |
ChIP experiment was carried out as previously described (Hawkins et al., 2010) with H3K9me3 (Abcam, ab8898) and H3K27m3(CST, 9733s) antibodies.
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
mapping to hg19 with bowtie2 deduplication by Picard macs2 callpeak --SPMR, and get pileup files Genome_build: hg19 Supplementary_files_format_and_content: bigwig, normalized pileup files
|
|
|
Submission date |
Dec 18, 2020 |
Last update date |
Dec 22, 2020 |
Contact name |
Xianglin Zhang |
E-mail(s) |
zhangxl.2015@tsinghua.org.cn
|
Organization name |
Shandong University
|
Department |
The Second Hospital of Shandong University
|
Lab |
Haiyang Guo Lab
|
Street address |
247 Beiyuan Street
|
City |
Jinan |
State/province |
Shandong |
ZIP/Postal code |
250033 |
Country |
China |
|
|
Platform ID |
GPL24676 |
Series (1) |
GSE133292 |
The loss of heterochromatin is associated with multiscale three-dimensional genome reorganization and aberrant transcription during cellular senescence |
|
Relations |
BioSample |
SAMN17117889 |
SRA |
SRX9704400 |