|
| Status |
Public on Jan 01, 2023 |
| Title |
FUUR1-RNAPol2 |
| Sample type |
SRA |
| |
|
| Source name |
human ASPS cell line
|
| Organism |
Homo sapiens |
| Characteristics |
chip antibody: POLII
cell line: FU-UR-1
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Lysates were clarified from sonicated nuclei and TARGET DNA complexes were isolated with antibody.
Library was prepared using NEBnext ChIP-seq libraray prep reagent set
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina MiSeq |
| |
|
| Data processing |
single 50bp sequencing on an Illumina MiSeq platform
ChIP-seq reads were aligned to the hg38 genome assembly using bowtie2 Version 2.3.4.2
peaks were called using MACS2 with following settings: -B --SPMR --pvalue=1e-2 --mfold 15 100
Genome_build: hg38
Supplementary_files_format_and_content: bigwig files were generated using multirep_macs2_pipeline.pl; Scores represent a normalized pileup signal
|
| |
|
| Submission date |
Dec 02, 2020 |
| Last update date |
Jan 01, 2023 |
| Contact name |
Jinxiu Li |
| Organization name |
China Agricutural University
|
| Department |
College of biological sciences
|
| Lab |
State key laboratories of Agrobiotechnology
|
| Street address |
Yuanmingyuan west road 2#
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100193 |
| Country |
China |
| |
|
| Platform ID |
GPL15520 |
| Series (2) |
| GSE162481 |
ASPSCR1-TFE3 reprograms transcription by organizing enhancers around hexameric VCP [Cell_lines_ChIP, FUUR1, FUUR1_ASPL_POLII] |
| GSE162609 |
ASPSCR1-TFE3 reprograms transcription by organizing enhancers around hexameric VCP |
|
| Relations |
| BioSample |
SAMN16977115 |
| SRA |
SRX9618637 |
