|
| Status |
Public on Mar 01, 2010 |
| Title |
Peripheral Whole Blood 102024 |
| Sample type |
genomic |
| |
|
| Source name |
genomic DNA from whole blood 102024
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: bi-sulphite converted genomic whole blood DNA from Case
agegroupatsampledraw: 65 to 70
ageatrecruitment: 69
ageatdiagnosis: 63
histology: Clear cell
stage: Ia
grade: Grade 2
pre-treatment sample: No
post-treatment sample: Yes
ca125: 26.6
batch: 9
beadchip_well: 4447820162_I
bs conversion c1: Grn 6296
bs conversion c2: Grn 6533
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted at Tepnel (www.tepnel.com) using a chloroform extraction method and 800ng DNA from each sample was bisulphite converted using the EZ DNA Methylation Kit D5008 (Zymo Research, Orange, CA, USA)
|
| Label |
as per Illumina Infinium 27k methylation assay protocol
|
| Label protocol |
A single base extension was performed using labelled DNP- and biotin labelled dNTPs. See the Illumina Infinium 27k methylation assay protocol.
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation27 Beadchip
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended scanner setting
|
| Description |
whole blood from cases are from postmenopausal women with ovarian cancer, drawn either before or after treatment
|
| Data processing |
BeadStudio software v3.2
|
| |
|
| Submission date |
Dec 31, 2009 |
| Last update date |
Mar 01, 2010 |
| Contact name |
Andrew E Teschendorff |
| E-mail(s) |
a.teschendorff@ucl.ac.uk
|
| Phone |
+44 02076790727
|
| Organization name |
University College London
|
| Department |
UCL Cancer Institute/Institute for Women's Health
|
| Street address |
72 Huntley Street
|
| City |
London |
| ZIP/Postal code |
WC1E 6BT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL8490 |
| Series (1) |
| GSE19711 |
Genome wide DNA methylation profiling of United Kingdom Ovarian Cancer Population Study (UKOPS) |
|
